Objectives/Goals
The purpose of my experiment is to determine if
elapsed time between crushing garlic and its usage will have an effect on
bacterial inhibition. My hypothesis is that as time intervals increase, zones
of inhibition will decrease.
Methods/Materials
Inoculum of Bacillus Atrophaeus TSB culture was
prepared for incubation. Bacteria were spread on six blood agar petri dishes to
ensure a confluent lawn of growth. 0.2 grams of crushed garlic was applied to petri
dish specimens at 15 min. time intervals, (0 - 60 min. and 24 hrs.)
Four petri
dishes became control groups. All were incubated overnight. Zones of inhibition
were measured and recorded.
Results
During the 1st experiment, no zones of inhibition
were observed. The next two experiments indicated an increase in zones of
inhibition. The 4th trial showed an increase in zones of inhibition during the
1st 30 minutes, followed by a gradual decrease.
Conclusions/Discussion
My hypothesis was correct; however, more time
intervals and experimental trials should be performed in order to establish
more accurate results.
Project Summary
My experiment is to determine if the elapsed time
between crushing garlic and its usage will have an effect upon bacterial
inhibition.
Project by Talar A. Alexanian
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